When Eliza Thistlethwaite was born on 15 December 1837, in Bradford, Yorkshire, England, United Kingdom, her father, John Thistlethwaite, was 46 and her mother, Esther Eliza Dickinson, was 30. She married Frederick Ashworth on 14 December 1859. They were the parents of at least 4 sons and 3 daughters. She lived in Bradford St James, Yorkshire, England, United Kingdom in 1861. She died on 23 March 1924, at the age of 86, and was buried in Toowong, Queensland, Australia.