When William Spencer was born on 11 October 1601, in Stotfold, Bedfordshire, England, his father, Gerrard Spencer I, was 25 and his mother, Alice Whitbread, was 23. He married Agnes Harris before 1633, in Cambridge, Middlesex, Massachusetts Bay Colony, British Colonial America. They were the parents of at least 1 son and 2 daughters. He died after 4 May 1640, in Hartford, Hartford, Connecticut Colony, British Colonial America, at the age of 38, and was buried in Hartford, Hartford, Connecticut, United States.