When Barnabas Lambert was born about 1751, in Hampshire, Virginia, United States, his father, John B. Lambert Sr, was 15 and his mother, Elizabeth Jeanette Henrich, was 13. He married Rebecca Rolls about 1773, in Hampshire, Virginia, United States. They were the parents of at least 2 sons and 3 daughters. He died before 6 August 1813, in Knox, Indiana, United States, at the age of 62.