When Ann Millhouse was born in 1772, in South Carolina, United States, her father, Henry Russel Millhouse, was 36 and her mother, Rebecca Cook, was 33. She married Amos Hawkins on 8 June 1797, in Union, South Carolina, United States. They were the parents of at least 3 sons and 5 daughters. She lived in Chester Township, Clinton, Ohio, United States in 1850. She died on 2 April 1855, in Clinton, Ohio, United States, at the age of 83, and was buried in Wayne Township, Warren, Ohio, United States.