When Johannes Hubertus Kwisthout was born on 3 November 1816, in Breda, North Brabant, Netherlands, his father, Johannes Kwisthout, was 28 and his mother, Anna Christina du Bois, was 20. He married Adriana Maria Ballintijn on 19 January 1843, in 's-Hertogenbosch, North Brabant, Netherlands. They were the parents of at least 3 sons and 2 daughters. He died on 2 March 1896, in his hometown, at the age of 79.