When Isaac Peet was born on 26 December 1800, in Stratford, Fairfield, Connecticut, United States, his father, John Peet, was 45 and his mother, Sarah Beers, was 43. He married Almira Chamberlain on 10 March 1825, in Cazenovia, Cazenovia, Madison, New York, United States. They were the parents of at least 3 sons. He died on 16 January 1839, in Eaton, Madison, New York, United States, at the age of 38, and was buried in Easton, Charlotte, New York, United States.