When Anna Stuart was born on 7 February 1813, in Deep River, Guilford, North Carolina, United States, her father, Jehu Stewart Sr., was 42 and her mother, Sarah Cook, was 40. She married Stewart Henry Aydelott on 10 November 1847, in Henry, Ripley Township, Rush, Indiana, United States. They were the parents of at least 2 sons and 2 daughters. She died on 11 August 1870, in Indiana, United States, at the age of 57.