When John Wesley Tweedy was born on 1 June 1891, in Campbell, Virginia, United States, his father, Emmett Filmore Tweedy, was 42 and his mother, Nancy Mae Jones, was 37. He married Annie Burton Cardwell on 23 December 1913, in Campbell, Albemarle, Virginia, United States. They were the parents of at least 3 sons and 1 daughter. He lived in Rustburg District, Campbell, Virginia, United States in 1940 and Concord, Jackson, Michigan, United States in 1958. He died on 22 July 1967, in Lynchburg, Virginia, United States, at the age of 76, and was buried in Concord, Campbell, Virginia, United States.