When Samuel Mattocks was born on 17 December 1687, in Boston, Suffolk, Massachusetts, United States, his father, Samuel Mattocks II, was 28 and his mother, Ann March, was 24. He married Admonition Tooker in 1712, in Boston, Suffolk, Massachusetts, United States. They were the parents of at least 3 sons and 3 daughters. He died on 27 December 1730, in his hometown, at the age of 43, and was buried in Riverside Cemetery, Middletown, Middlesex, Connecticut, United States.